Isolation of DNA fragments from polyacrylamide gels by the crush and soak method.
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چکیده
منابع مشابه
Microsatellite (SSR) amplification by PCR usually led to polymorphic bands: Evidence which shows replication slippage occurs in extend or nascent DNA strands
Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...
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similar yield as the standard method. Therefore, using this modified method, we can reduce the soaking time from 15 h to about 10 min. In summary, a simple modification of the crush-and-soak method allows the recovery of ODN from polyacrylamide gel within 30 min with high yield. The key difference in our procedure is the replacement of the overnight soak with a heat/freeze step (summarized in T...
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Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...
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Sons, 1989:3.2. 5. Chomczynski P, Sacchi H. Single-step method of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156–9. 6. Bauer D, Warthoe P, Rohde M, Strauss M. Detection and differential display of expressed genes by DDRT-PCR. PCR Methods Appl 1994;4:S97–108. 7. Bassam B, Caetano-Anolles G, Gresshoff P. Fast and sensitive silver staining of ...
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ورودعنوان ژورنال:
- CSH protocols
دوره 2006 1 شماره
صفحات -
تاریخ انتشار 2006